SEQUENCING
Long-read sequencing (PacBio HiFi) Reads single DNA molecules thousands of bases long at 99.9% accuracy, and detects methylation in the same run.
hairpin adapters 99.9% accurate 15–20 kb 1 DNA closed into a circle 2 Polymerase reads it many times 3 Errors fall at random places 4 Consensus: one HiFi read hairpin adapters 99.9% accurate 15–20 kb 1 DNA closed into a circle 2 Polymerase reads it many times 3 Errors fall at random places 4 Consensus: one HiFi read hairpin adapters 99.9% accurate 15–20 kb 1 DNA closed into a circle 2 Polymerase reads it many times 3 Errors fall at random places 4 Consensus: one HiFi read hairpin adapters 99.9% accurate 15–20 kb 1 DNA closed into a circle 2 Polymerase reads it many times 3 Errors fall at random places 4 Consensus: one HiFi read The same circular molecule is read many times; random errors cancel out, leaving one long, 99.9% accurate HiFi read. Whole genomes and de novo assembly · Structural variants and repeat expansions · Full-length RNA isoforms and 16S microbiome
PacBio Revio and Vega systems, HiFi kits, Kinnex, PureTarget (official distributor) Yourgene Health LightBench size selection for long-read libraries Browse products → SEQUENCING
Short-read sequencing kits Massively parallel sequencing of short fragments (about 150-300 bp): the lowest cost per base for counting and variant calling.
flow cell A C G T cycle by cycle 1 Fragment DNA, add adapters 2 Copies form clusters 3 Read one base per cycle 4 Align millions of short reads flow cell A C G T cycle by cycle 1 Fragment DNA, add adapters 2 Copies form clusters 3 Read one base per cycle 4 Align millions of short reads flow cell A C G T cycle by cycle 1 Fragment DNA, add adapters 2 Copies form clusters 3 Read one base per cycle 4 Align millions of short reads flow cell A C G T cycle by cycle 1 Fragment DNA, add adapters 2 Copies form clusters 3 Read one base per cycle 4 Align millions of short reads Short fragments are copied into clusters and read one base per cycle, by colour. Exomes and gene panels · Liquid biopsy and ctDNA · RNA-seq and large cohorts
Illumina, MGI, GeneMind and Thermo Fisher Sequencing kits for these platforms PacBio Onso short-read system and chemistry Twist Bioscience Library prep and exome kits for short-read platforms Browse products → SEQUENCING
Nanopore sequencing kits Ultra-long reads from DNA passing through a protein pore; portable and real-time.
motor protein membrane current (pA) ACGTTAGC… in real time 1 Adapter with motor protein 2 Strand threads through a pore 3 Bases change the current 4 Signal decoded to sequence motor protein membrane current (pA) ACGTTAGC… in real time 1 Adapter with motor protein 2 Strand threads through a pore 3 Bases change the current 4 Signal decoded to sequence motor protein membrane current (pA) ACGTTAGC… in real time 1 Adapter with motor protein 2 Strand threads through a pore 3 Bases change the current 4 Signal decoded to sequence motor protein membrane current (pA) ACGTTAGC… in real time 1 Adapter with motor protein 2 Strand threads through a pore 3 Bases change the current 4 Signal decoded to sequence DNA is pulled through a protein pore; each stretch of bases changes the current in its own way. Field and outbreak sequencing · Rapid pathogen identification
4bases Oxford Nanopore kits supplied through 4bases Browse products → SEQUENCING
Sanger sequencing and oligonucleotides Gold-standard confirmation of single variants, plus custom primers, probes and modified oligos.
stop bases laser G A T C A G 1 Primer, polymerase, dye stop bases 2 Copies end at every position 3 Capillary sorts by length 4 Colour peaks give the sequence stop bases laser G A T C A G 1 Primer, polymerase, dye stop bases 2 Copies end at every position 3 Capillary sorts by length 4 Colour peaks give the sequence stop bases laser G A T C A G 1 Primer, polymerase, dye stop bases 2 Copies end at every position 3 Capillary sorts by length 4 Colour peaks give the sequence stop bases laser G A T C A G 1 Primer, polymerase, dye stop bases 2 Copies end at every position 3 Capillary sorts by length 4 Colour peaks give the sequence Copies stop at every base with a coloured end; sorting them by length spells out the sequence. Variant confirmation · Clone verification · Primers and probes for PCR and qPCR
Microsynth Sanger and NGS services; research, diagnostic and modified oligos Browse products → LIBRARY PREP
Target enrichment and library preparation Focuses sequencing on the genes that matter, by hybridisation capture or multiplex amplicons.
B B biotin probes magnet 1 Library of all fragments 2 Probes bind target genes 3 Beads pull targets out 4 Only targets are sequenced B B biotin probes magnet 1 Library of all fragments 2 Probes bind target genes 3 Beads pull targets out 4 Only targets are sequenced B B biotin probes magnet 1 Library of all fragments 2 Probes bind target genes 3 Beads pull targets out 4 Only targets are sequenced B B biotin probes magnet 1 Library of all fragments 2 Probes bind target genes 3 Beads pull targets out 4 Only targets are sequenced Hybridisation capture keeps only the genes you want, so sequencing is spent on targets. Exomes and custom panels · Oncology and hereditary-cancer panels · Methylation panels
Browse products → CLINICAL GENOMICS
Clinical NGS and genetic testing assays Ready-to-run, CE-IVD and research assays with analysis and reporting for diagnostic labs.
1 Blood, tissue or FFPE 2 Library with a validated panel 3 Sequencing 4 Variants called, report issued 1 Blood, tissue or FFPE 2 Library with a validated panel 3 Sequencing 4 Variants called, report issued 1 Blood, tissue or FFPE 2 Library with a validated panel 3 Sequencing 4 Variants called, report issued 1 Blood, tissue or FFPE 2 Library with a validated panel 3 Sequencing 4 Variants called, report issued A validated kit takes a patient sample through library, sequencing and software to a report. Solid-tumour, HRD and fusion testing · NIPT and reproductive health · Pharmacogenomics · HIV and HCV drug-resistance genotyping
Browse products → MOLECULAR BIOLOGY
PCR, RT-PCR and qPCR Copies and measures DNA and RNA: from routine genotyping to high-fidelity cloning and quantitative gene expression.
RNA cDNA RT-PCR only 95 °C ~60 °C 72 °C threshold cycles → 1 RNA → cDNA (reverse transcription) 2 Denature: strands separate 3 Anneal: primers bind 4 Extend: copies double each cycle 5 qPCR: fluorescence rises with copies RNA cDNA RT-PCR only 95 °C ~60 °C 72 °C threshold cycles → 1 RNA → cDNA (reverse transcription) 2 Denature: strands separate 3 Anneal: primers bind 4 Extend: copies double each cycle 5 qPCR: fluorescence rises with copies RNA cDNA RT-PCR only 95 °C ~60 °C 72 °C threshold cycles → 1 RNA → cDNA (reverse transcription) 2 Denature: strands separate 3 Anneal: primers bind 4 Extend: copies double each cycle 5 qPCR: fluorescence rises with copies RNA cDNA RT-PCR only 95 °C ~60 °C 72 °C threshold cycles → 1 RNA → cDNA (reverse transcription) 2 Denature: strands separate 3 Anneal: primers bind 4 Extend: copies double each cycle 5 qPCR: fluorescence rises with copies RNA cDNA RT-PCR only 95 °C ~60 °C 72 °C threshold cycles → 1 RNA → cDNA (reverse transcription) 2 Denature: strands separate 3 Anneal: primers bind 4 Extend: copies double each cycle 5 qPCR: fluorescence rises with copies Each PCR cycle doubles the target; RT-PCR adds a first step that copies RNA into cDNA, and qPCR measures the product as it forms. Genotyping and cloning · Gene-expression qPCR · Pathogen and GMO detection
Takara Bio PrimeSTAR, Ex Taq, LA Taq, Terra Direct, PrimeScript and TB Green qPCR AM Diagnostics Ready-to-use real-time PCR detection kits for infections, genetics and AMR BAG Diagnostics HLA and blood-group typing by real-time PCR, SSO and SSP; fetal RhD Allsheng Real-time PCR systems Shentek Bio 96S real-time PCR system Browse products → MOLECULAR BIOLOGY
Isothermal amplification Amplifies nucleic acid at one temperature, so testing needs little equipment.
60–65 °C, constant strand displacement minutes, not hours + – 1 One temperature, no thermal cycler 2 Polymerase peels and copies 3 Billions of copies 4 Read colour or fluorescence 60–65 °C, constant strand displacement minutes, not hours + – 1 One temperature, no thermal cycler 2 Polymerase peels and copies 3 Billions of copies 4 Read colour or fluorescence 60–65 °C, constant strand displacement minutes, not hours + – 1 One temperature, no thermal cycler 2 Polymerase peels and copies 3 Billions of copies 4 Read colour or fluorescence 60–65 °C, constant strand displacement minutes, not hours + – 1 One temperature, no thermal cycler 2 Polymerase peels and copies 3 Billions of copies 4 Read colour or fluorescence One constant temperature and a strand-displacing polymerase: no thermal cycler needed. Rapid pathogen detection · Point-of-need testing
Browse products → GENE FUNCTION
Cloning, gene editing and gene delivery Builds DNA constructs, edits genomes with CRISPR and delivers genes into cells.
small indel new sequence Cas9 + guide double-strand cut 1 Guide RNA finds the target 2 Cas9 cuts both strands 3 Repair errors: knock-out 4 Donor template: knock-in small indel new sequence Cas9 + guide double-strand cut 1 Guide RNA finds the target 2 Cas9 cuts both strands 3 Repair errors: knock-out 4 Donor template: knock-in small indel new sequence Cas9 + guide double-strand cut 1 Guide RNA finds the target 2 Cas9 cuts both strands 3 Repair errors: knock-out 4 Donor template: knock-in small indel new sequence Cas9 + guide double-strand cut 1 Guide RNA finds the target 2 Cas9 cuts both strands 3 Repair errors: knock-out 4 Donor template: knock-in Cas9 cuts where the guide RNA matches; the cell's repair makes the edit. Seamless cloning · CRISPR knock-outs and knock-ins · Lentiviral delivery and inducible expression
Takara Bio In-Fusion, Guide-it CRISPR, Lenti-X, Tet-On 3G Browse products → IMMUNOASSAYS
ELISA, CLIA and multiplex immunoassays Measures proteins, hormones, cytokines and antibodies in serum, plasma and culture supernatants.
colour 1 Capture antibody coats the well 2 Antigen in the sample binds 3 Enzyme-labelled antibody binds 4 Substrate turns colour: read OD colour 1 Capture antibody coats the well 2 Antigen in the sample binds 3 Enzyme-labelled antibody binds 4 Substrate turns colour: read OD colour 1 Capture antibody coats the well 2 Antigen in the sample binds 3 Enzyme-labelled antibody binds 4 Substrate turns colour: read OD colour 1 Capture antibody coats the well 2 Antigen in the sample binds 3 Enzyme-labelled antibody binds 4 Substrate turns colour: read OD In a sandwich ELISA the colour is proportional to the amount of antigen in the sample. Biomarker and cytokine measurement · Serology · Allergen and residue testing
Browse products → IMMUNOASSAYS
ELISpot and FluoroSpot Counts individual cytokine-secreting T cells, the most sensitive measure of antigen-specific immunity.
1 Antibody-coated membrane 2 Cells secrete cytokine 3 Cells removed; spots developed 4 Each spot is one active cell 1 Antibody-coated membrane 2 Cells secrete cytokine 3 Cells removed; spots developed 4 Each spot is one active cell 1 Antibody-coated membrane 2 Cells secrete cytokine 3 Cells removed; spots developed 4 Each spot is one active cell 1 Antibody-coated membrane 2 Cells secrete cytokine 3 Cells removed; spots developed 4 Each spot is one active cell ELISpot counts single responding cells, so it detects very rare T-cell responses. Vaccine and TB immunity studies · Transplant and infection monitoring
Mabtech ELISpot and FluoroSpot kits, peptide pools and readers Browse products → IMMUNOASSAYS
Serology: blood groups and HLA Antibodies in an antiserum bind matching antigens on red cells or lymphocytes, so the cells clump or are killed: the basis of blood grouping and HLA serology.
antigen A anti-A serum clumped: group A smooth: no match 1 Cells carry antigens 2 Add antibody (antiserum) 3 Match: cells agglutinate 4 No match: cells stay apart antigen A anti-A serum clumped: group A smooth: no match 1 Cells carry antigens 2 Add antibody (antiserum) 3 Match: cells agglutinate 4 No match: cells stay apart antigen A anti-A serum clumped: group A smooth: no match 1 Cells carry antigens 2 Add antibody (antiserum) 3 Match: cells agglutinate 4 No match: cells stay apart antigen A anti-A serum clumped: group A smooth: no match 1 Cells carry antigens 2 Add antibody (antiserum) 3 Match: cells agglutinate 4 No match: cells stay apart The antigen–antibody reaction behind ABO and RhD grouping, crossmatching and HLA serology. ABO and RhD grouping · HLA serology and lymphocytotoxicity · HLA antibody detection
BAG Diagnostics Blood-group serology sera, HISTO TRAY HLA serology, HISTO SPOT HLA AB and SeraScreen Abs Browse products → CELL ANALYSIS
Flow cytometry and cell-based assays Identifies and counts cell types by surface and intracellular markers; measures cell health and function.
laser 1 Label cells with fluorescent Abs 2 Cells pass a laser one by one 3 Detectors read scatter & colour 4 Plot separates cell types laser 1 Label cells with fluorescent Abs 2 Cells pass a laser one by one 3 Detectors read scatter & colour 4 Plot separates cell types laser 1 Label cells with fluorescent Abs 2 Cells pass a laser one by one 3 Detectors read scatter & colour 4 Plot separates cell types laser 1 Label cells with fluorescent Abs 2 Cells pass a laser one by one 3 Detectors read scatter & colour 4 Plot separates cell types Every cell is measured individually, thousands per second. Immunophenotyping · Apoptosis and proliferation · Potency and functional bioassays
Browse products → PROTEIN ANALYSIS
Quantitative Western blot and imaging Detects and measures specific proteins with fluorescent imaging that stays linear across a wide range.
big small current 1 Gel separates by size 2 Transfer to membrane 3 Antibodies find the target 4 Image and quantify bands big small current 1 Gel separates by size 2 Transfer to membrane 3 Antibodies find the target 4 Image and quantify bands big small current 1 Gel separates by size 2 Transfer to membrane 3 Antibodies find the target 4 Image and quantify bands big small current 1 Gel separates by size 2 Transfer to membrane 3 Antibodies find the target 4 Image and quantify bands Size separation plus antibody specificity identifies one protein in a mixture. Protein expression · In-Cell Western assays · Gel and blot imaging
Browse products → CELL BIOLOGY
Cell culture, primary cells and stem cells Media, sera, authenticated cell lines and stem-cell systems for reproducible cell work.
vial tissue medium + serum 37 °C · 5% CO₂ 1 Isolate cells or thaw a cell line 2 Seed a flask with medium 3 Grow in the incubator 4 Passage when confluent: split vial tissue medium + serum 37 °C · 5% CO₂ 1 Isolate cells or thaw a cell line 2 Seed a flask with medium 3 Grow in the incubator 4 Passage when confluent: split vial tissue medium + serum 37 °C · 5% CO₂ 1 Isolate cells or thaw a cell line 2 Seed a flask with medium 3 Grow in the incubator 4 Passage when confluent: split vial tissue medium + serum 37 °C · 5% CO₂ 1 Isolate cells or thaw a cell line 2 Seed a flask with medium 3 Grow in the incubator 4 Passage when confluent: split Healthy, contamination-free cultures depend on good medium, serum, plastics and authenticated cells. Cell-line and primary-cell culture · Stem-cell maintenance and differentiation · Bioproduction
Browse products → BIOPHARMA QC
Biopharma quality control Release testing for biologics, vaccines and cell and gene therapies.
batch sample host-cell DNA 1 Product sample 2 Residual DNA by qPCR 3 Mycoplasma absent 4 Potency met: batch released batch sample host-cell DNA 1 Product sample 2 Residual DNA by qPCR 3 Mycoplasma absent 4 Potency met: batch released batch sample host-cell DNA 1 Product sample 2 Residual DNA by qPCR 3 Mycoplasma absent 4 Potency met: batch released batch sample host-cell DNA 1 Product sample 2 Residual DNA by qPCR 3 Mycoplasma absent 4 Potency met: batch released Typical release tests for biologics, vaccines and cell and gene therapies. Residual host-cell DNA · Mycoplasma detection · Potency assays and reference standards
Browse products → AGRI-FOOD AND VETERINARY
Food, feed and veterinary testing ELISA and real-time PCR kits for animal disease, food safety and authenticity.
milk feed ELISA real-time PCR 1 Milk, feed, tissue or swab 2 Antibodies, toxins or residues 3 Pathogen DNA or RNA 4 Herd health and food-safety call milk feed ELISA real-time PCR 1 Milk, feed, tissue or swab 2 Antibodies, toxins or residues 3 Pathogen DNA or RNA 4 Herd health and food-safety call milk feed ELISA real-time PCR 1 Milk, feed, tissue or swab 2 Antibodies, toxins or residues 3 Pathogen DNA or RNA 4 Herd health and food-safety call milk feed ELISA real-time PCR 1 Milk, feed, tissue or swab 2 Antibodies, toxins or residues 3 Pathogen DNA or RNA 4 Herd health and food-safety call The same two workhorses, ELISA and real-time PCR, cover most animal-health and food-safety testing. Poultry, livestock and aquaculture disease · Mycotoxins and drug residues · Meat species and GMO identification
Browse products → CONSUMABLES
Laboratory plastics and filtration Certified tips, tubes, plates and filters that keep PCR and cell culture free of contamination.
aerosol filter 1 Pipetting makes aerosols 2 Filter traps them 3 No carry-over between samples aerosol filter 1 Pipetting makes aerosols 2 Filter traps them 3 No carry-over between samples aerosol filter 1 Pipetting makes aerosols 2 Filter traps them 3 No carry-over between samples Filter tips stop the aerosols that cause false positives in PCR. PCR and qPCR set-up · Cell culture · Sample storage and filtration
JET BIOFIL Filter tips, PCR plastics, culture vessels and filters Browse products → 