One pipeline, several suppliers

The 4bases ONCO4B-IVD route replaces library prep, enrichment and reporting with one CE-IVD kit and its own software.

Pair with the tumour HRD workflow when PARP-inhibitor eligibility is the clinical question.

For full-length fusion transcripts, see the long-read Iso-Seq workflow.

Low-frequency variant calling needs UMI-aware analysis and deep coverage; we size read depth with you before ordering.

Unsolved cases can move to HiFi whole-genome sequencing to catch structural variants and repeats that exomes miss.

Short reads cannot span most pathogenic expansions; this is a long-read-only application.

Real-time PCR gives the fastest typing; add SSO or SSP to resolve ambiguities, and long-read HiFi when allele-level resolution is needed.

Serology answers most routine questions; genotyping settles weak D, partial D and patients whose red cells are coated or recently transfused.

Also used for agrigenomics: crop and livestock reference genomes and pan-genomes.

HLA amplicon primers and typing software are chosen with you; neither is a PacBio catalogue kit.

The two routes are alternatives: choose NGS for scale, digital PCR for low-volume labs without a sequencer.

Stages are independent test types; labs usually adopt the ones matching their menu.

Run serology before and after vaccination to check titres; send PCR only on suspect birds to keep costs down.

For in-house primer design across more species, see JBS's species-ID primer designer.

For new crop reference genomes, use the PacBio HiFi whole-genome workflow.

Applies equally to tumour, gut, soil and rumen samples.